Sprite Cow is described as 'helps you get the background-position, width and height of sprites within a spritesheet as a nice bit of copyable css' and is an website in the Development category. There are five alternatives to Sprite Cow, not only websites but also apps for Windows. The best alternative is CSS Sprite Generator, which is both free and Open Source. Other great sites and apps similar to Sprite Cow are SpritePad (Free), SpriteSheep (Freemium), Spritesheet (Free) and CSS Sprite Generator (Beta) (Free).
Optimized for speed: No download, no setup, no long-winded uploads. SpritePad works hassle-free directly in your browser, just drag & drop your images and you can immediately work with them to create your unique CSS sprites.