BenchCalc

CALCULATORS Nucleic acid mass/moles, A260 concentration, Tm prediction with primer pair comparison and dimer checking. Protein molecular weight from sequence, Bradford/BCA assay with standard curve or quick estimate mode. Peptide isoelectric point and charge calculator, per-moiety charges, and charge-vs-pH curve. Dilution, serial dilution, molarity, Beer-Lambert A280. Buffer preparation, percent solutions, normality with equivalence factors and N1V1=N2V2 dilution, resuspension. Cell counting and seeding, centrifuge g/RPM conversion, doubling time, radioactive decay with preset isotopes, reference date or elapsed time input, activity unit conversion, and decay curve. PCR master mix with enzyme presets, template DNA concentration helper and 10% overage. Restriction digest calculator: select up to 3 enzymes from the built-in database with automatic buffer compatibility checking, methylation and star activity warnings, and save/recall setups. Sequence translator: DNA, RNA, and protein conversion with auto-detection, reverse complement, six reading frames with ORF highlighting, and feature annotation. Ligation calculator with insert:vector molar ratios, enzyme presets and full reaction volume breakdown. General reaction mix: define custom components with C1V1=C2V2 scaling. Unit converter with full SI magnitude ladder (femto to peta) across 7 categories. Quick calculator & Lab timer.

PROTOCOLS 11 step-through lab protocols: miniprep, ethanol precipitation, gel extraction, PCR cleanup, restriction digest, ligation, heat shock transformation, E. coli growth & harvest, affinity column purification, column maintenance, and inclusion body purification with urea/guanidine solubilisation routes and refolding screen guide. Each protocol has checklist and focus modes, duration badges, volume chips, warnings, and tips. Progress is saved per protocol.

REFERENCE TABLES 20 amino acids with chemical structures, MW, pI, pKa values, pH-dependent protonation states, per-moiety charge display, and pH slider. 5 nucleotide bases with structures and IUPAC degenerate codes. 64 codons — standard genetic code, searchable. 132 restriction enzymes: Type II, IIS, nicking, and homing endonucleases with methylation sensitivity, star activity warnings, buffer info, and compatible end matching. Filter by type, overhang, or sort by name, site length, or buffer. 284 common lab reagents: MW, formula, CAS, working concentrations, storage temperature, solubility, and hazard warnings. Row and grid view toggle. Sort by name or molecular weight.

FEATURES Dark and light themes with auto mode. Three text sizes. Favourites and category filtering. Chemical structure rendering for amino acids and nucleotides. Cross-calculator clipboard. Smart custom keypad with numeric, nucleotide, and amino acid modes. Optional haptic feedback. Works completely offline. No account required, no data collected.

Built by a scientist, for scientists. One purchase, everything included.